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HAEMAROXYLIN MAYER’S SOLUTION |
| Article No. | 83760 | Grade | For Microscopical Staining | |
| Synonyms | Hemalum mayer’s | C.I. No. | — | |
| CAS No. | — | Purity | — | |
| Molecular Formula | — | Molecular Weight | — | |
| H.S. Code | 38220090 | Shelf Life | 36 Months | |
| Physical Properties | ||||
| Physical state at 20 °C | Liquid | Colour | Red-brown | |
| Solubility | Miscible with water. | |||
| Specifications Parameters | ||||
| Description | Red-brown solution. | |||
| Suitability for microscopy | Passes test | |||
| Directions | A. Nuclear counter stain for Immunohistochemical staining :
1. Complete individual staining procedure (as desired). Rinse the slide with deionized water. 2. Stain the tissue section or the cell preparation with haematoxylin for 30-60 seconds. 3. Rinse with water to remove excess reagent. 4. Place in bluing reagent (alkaline solution such as a weak ammonia solution, 0.08% in water) until stain is blue (approximately 30 seconds). 5. Rinse in deionized water. 6. Section can be mounted in aqueous mounting media. B. Haematoxylin and Eosin staining : 1. Prepare 95% alcohol solution. 2. Deparaffinize the tissue section and hydrate to water or fix and hydrate frozen sections. 3. Stain tissue section or cell preparation for 30-60 seconds with haematoxylin. 4. Rinse with water to remove excess reagent. 5. Place in bluing reagent until the stain is blue. 6. Rinse in deionized water. 7. If alcoholic eosin is used, place slide in 95% alcohol for 30 seconds. 8. Place eosin counter stain for 30-60 seconds. 9. Dehydrate in two changes each of reagent 95% alcohol, absolute alcohol and xylene for 2 minutes each. 1. 10. Mount with synthetic mounting medium and examine the slide under microscope. |
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| Results | Nuclei : Blue colour | |||
| Intended Use | Haematoxylin (Mayer’s) is recommended for Immunohistochemical and cytochemical Staining (as Nuclear Counter Stain) (PAS Staining Procedure). It may also be used for routine Haematoxylin and Eosin Staining. | |||
| Principle And Interpretation | Haematoxylin is extracted from logwood with hot water, and then precipitated out from the aqueous solution using urea. Haematoxylin itself is not a stain. Its major oxidation product is Haematein that is a natural dye responsible for colour properties. Hematein can be produced by natural oxidation on exposure to light and air. Ehrlichs and Delafields haematoxylin solutions are examples of naturally ripened haematoxylins. Chemical oxidation uses sodium iodate (e.g., Mayer’s haematoxylin) or mercuric oxide (e.g., Harris haematoxylin). Hematein is anionic, having poor affinity for tissue, and is inadequate as a nuclear stain without the presence of a mordant. The mordents used are salts of aluminum, iron and tungsten. Mayer’s
haematoxylin is alum haematoxylin, chemically ripened with sodium iodate. It can be used as a regressive stain like any alum haematoxylin. However, it is also useful as a progressive stain, particularly in situations where a nuclear counterstain is needed in the demonstration of glycogen, in various enzyme histochemical techniques. Haematoxylin and eosin are the principle stains used for the demonstration of nucleus and the cytoplasmic inclusions. Here, acid reacting components of the cell combine with alkaline dyes and the alkaline area react with acid dyes. |
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| Note(s): Assay (if applicable) method mentioned.
WARNING Hazard statements: May cause respiratory irritation. Causes skin irritation. Causes serious eye irritation. Precautionary statements Prevention: Avoid breathing dust/fume/gas/mist/vapours/spray. Wash thoroughly after handling. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. Response: IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If eye irritation persists: Get medical advice/attention. |
IMDG Code : —
UN No. : — IATA : — |
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| Disposal: Dissolve the chemical to be disposed, in water and allow it to run to waste, diluting with large quantities of water. The quantities greater than 10g should be dissolved in water and transferred to heavy metal waste drums for collection by specialist disposal company. | ||||
| Hazard Pictogram(s) — GHS07 | ||||
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